Isolation of fla-lacZ fusions in Escherichia coli K-12: most fusions result in soluble beta-galactosidase.
A series of fusions of flagellar genes to the lacZ gene was generated by insertion of Mu dII301 (Apr lac) bacteriophage into the genome of Escherichia coli. The beta-galactosidase activity in each resulting mutant was measured, and the location of the activity in the membrane, periplasmic, or cytopl...
Guardat en:
| Publicat a: | J Bacteriol |
|---|---|
| Autor principal: | |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1988
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211065/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3127385/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.170.4.1980-1983.1988 |
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