Use of gene replacement to construct Escherichia coli strains carrying mutations in two genes required for stability of multicopy plasmids.
Escherichia coli mutants completely defective in ColE1 cer-mediated site-specific recombination have been mapped to two genes, xerA and xerB. In this study, xerA xerB double mutants were constructed by gene replacement with a lambda dv plasmid and were shown to be both viable and defective in ColE1...
Guardat en:
| Publicat a: | J Bacteriol |
|---|---|
| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1989
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209887/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2649493/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.4.2241-2243.1989 |
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