Use of gene replacement to construct Escherichia coli strains carrying mutations in two genes required for stability of multicopy plasmids.

Escherichia coli mutants completely defective in ColE1 cer-mediated site-specific recombination have been mapped to two genes, xerA and xerB. In this study, xerA xerB double mutants were constructed by gene replacement with a lambda dv plasmid and were shown to be both viable and defective in ColE1...

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Détails bibliographiques

Publié dans:	J Bacteriol
Auteurs principaux:	Flinn, H, Burke, M, Stirling, C J, Sherratt, D J
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1989
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209887/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2649493/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.4.2241-2243.1989
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