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Direct observation of the reversible unwinding of a single DNA molecule caused by the intercalation of ethidium bromide

Ethidium bromide (EtBr) is the conventional intercalator for visualizing DNA. Previous studies suggested that EtBr lengthens and unwinds double-stranded DNA (dsDNA). However, no one has observed the unwinding of a single dsDNA molecule during intercalation. We developed a simple method to observe th...

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Detalhes bibliográficos
Main Authors: Hayashi, Masahito, Harada, Yoshie
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2095801/
https://ncbi.nlm.nih.gov/pubmed/17905818
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm529
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