Cloning, sequencing, and expression in Escherichia coli of a Streptococcus faecalis autolysin.

A Streptococcus faecalis genomic bank was obtained by partial digestion with MboI and cloning into the SalI restriction site of pTZ18R. Screening of about 60,000 Escherichia coli transformants for cell wall lysis activity was done by exposing recombinant colonies grown on medium containing lyophiliz...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Béliveau, C, Potvin, C, Trudel, J, Asselin, A, Bellemare, G
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC208289/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1679432/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.18.5619-5623.1991
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