Cloning of an Intracellular Poly[d(−)-3-Hydroxybutyrate] Depolymerase Gene from Ralstonia eutropha H16 and Characterization of the Gene Product

An intracellular poly[d(−)-3-hydroxybutyrate] (PHB) depolymerase gene (phaZ) has been cloned from Ralstonia eutropha H16 by the shotgun method, sequenced, and characterized. Nucleotide sequence analysis of a 2.3-kbp DNA fragment revealed an open reading frame of 1,260 bp, encoding a protein of 419 a...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Saegusa, Haruhisa, Shiraki, Mari, Kanai, Chie, Saito, Terumi
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2001
Assuntos:	Enzymes and Proteins
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC94854/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11114905/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JB.183.1.94-100.2001
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