Cloning, sequencing, and expression in Escherichia coli of a Streptococcus faecalis autolysin.

A Streptococcus faecalis genomic bank was obtained by partial digestion with MboI and cloning into the SalI restriction site of pTZ18R. Screening of about 60,000 Escherichia coli transformants for cell wall lysis activity was done by exposing recombinant colonies grown on medium containing lyophiliz...

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Pubblicato in:	J Bacteriol
Autori principali:	Béliveau, C, Potvin, C, Trudel, J, Asselin, A, Bellemare, G
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1991
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC208289/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1679432/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.18.5619-5623.1991
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