Transposon Tn5supF-based reverse genetic method for mutational analysis of Escherichia coli with DNAs cloned in lambda phage.

An efficient method for systematic mutational analysis of the Escherichia coli genome was developed. It entails Tn5supF transposition to lambda-E. coli hybrid phage clones (Kohara library) and then transduction of recipient cells to Sup+. Essential and nonessential genes are distinguished by the abi...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Phadnis, S H, Kulakauskas, S, Krishnan, B R, Hiemstra, J, Berg, D E
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC207086/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1846153/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.2.896-899.1991
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