Transposon Tn5supF-based reverse genetic method for mutational analysis of Escherichia coli with DNAs cloned in lambda phage.

An efficient method for systematic mutational analysis of the Escherichia coli genome was developed. It entails Tn5supF transposition to lambda-E. coli hybrid phage clones (Kohara library) and then transduction of recipient cells to Sup+. Essential and nonessential genes are distinguished by the abi...

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Bibliografische gegevens

Gepubliceerd in:	J Bacteriol
Hoofdauteurs:	Phadnis, S H, Kulakauskas, S, Krishnan, B R, Hiemstra, J, Berg, D E
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	American Society for Microbiology (ASM) 1991
Onderwerpen:	Research Article
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC207086/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1846153/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.173.2.896-899.1991
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