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Excess intracellular concentration of the pSC101 RepA protein interferes with both plasmid DNA replication and partitioning.

RepA, a plasmid-encoded gene product required for pSC101 replication in Escherichia coli, is shown here to inhibit the replication of pSC101 in vivo when overproduced 4- to 20-fold in trans. Unlike plasmids whose replication is prevented by mutations in the repA gene, plasmids prevented from replica...

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Udgivet i:J Bacteriol
Principais autores: Ingmer, H, Cohen, S N
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1993
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206959/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8253672/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.175.24.7834-7841.1993
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