Construction of recombination-deficient strains of Streptococcus gordonii by disruption of the recA gene.

Degenerate oligonucleotide primers were used in a polymerase chain reaction (PCR) to amplify a region of the recA sequence of Streptococcus gordonii Challis. The resulting PCR fragment was cloned into the suicide vector pAM6199 and introduced into strain Challis, giving rise to recombination-deficie...

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Main Authors: Vickerman, M M, Heath, D G, Clewell, D B
Format: Artigo
Jezik:Inglês
Izdano: 1993
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC206735/
https://ncbi.nlm.nih.gov/pubmed/8407809
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