Construction of recombination-deficient strains of Streptococcus gordonii by disruption of the recA gene.

Degenerate oligonucleotide primers were used in a polymerase chain reaction (PCR) to amplify a region of the recA sequence of Streptococcus gordonii Challis. The resulting PCR fragment was cloned into the suicide vector pAM6199 and introduced into strain Challis, giving rise to recombination-deficie...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Vickerman, M M, Heath, D G, Clewell, D B
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1993
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206735/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8407809/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.175.19.6354-6357.1993
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