Construction of recombination-deficient strains of Streptococcus gordonii by disruption of the recA gene.
Degenerate oligonucleotide primers were used in a polymerase chain reaction (PCR) to amplify a region of the recA sequence of Streptococcus gordonii Challis. The resulting PCR fragment was cloned into the suicide vector pAM6199 and introduced into strain Challis, giving rise to recombination-deficie...
Guardado en:
| Publicado en: | J Bacteriol |
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| Autores principales: | , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1993
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC206735/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8407809/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.175.19.6354-6357.1993 |
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