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Efficient transposition in mycobacteria: construction of Mycobacterium smegmatis insertional mutant libraries.

The Tn611 transposon was inserted into pCG63, a temperature-sensitive plasmid isolated from an Escherichia coli-mycobacterial shuttle vector which contains the pAL5000 and pUC18 replicons. The resulting plasmid, pCG79, was used to generate a large number of insertional mutations in Mycobacterium sme...

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Udgivet i:J Bacteriol
Principais autores: Guilhot, C, Otal, I, Van Rompaey, I, Martìn, C, Gicquel, B
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1994
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC205082/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8288551/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.176.2.535-539.1994
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