Efficient transposition in mycobacteria: construction of Mycobacterium smegmatis insertional mutant libraries.

The Tn611 transposon was inserted into pCG63, a temperature-sensitive plasmid isolated from an Escherichia coli-mycobacterial shuttle vector which contains the pAL5000 and pUC18 replicons. The resulting plasmid, pCG79, was used to generate a large number of insertional mutations in Mycobacterium sme...

Popoln opis

Shranjeno v:
Bibliografske podrobnosti
Main Authors: Guilhot, C, Otal, I, Van Rompaey, I, Martìn, C, Gicquel, B
Format: Artigo
Jezik:Inglês
Izdano: 1994
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC205082/
https://ncbi.nlm.nih.gov/pubmed/8288551
Oznake: Označite
Brez oznak, prvi označite!

Podobne knjige/članki