Efficient transposition in mycobacteria: construction of Mycobacterium smegmatis insertional mutant libraries.
The Tn611 transposon was inserted into pCG63, a temperature-sensitive plasmid isolated from an Escherichia coli-mycobacterial shuttle vector which contains the pAL5000 and pUC18 replicons. The resulting plasmid, pCG79, was used to generate a large number of insertional mutations in Mycobacterium sme...
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| Publicado en: | J Bacteriol |
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| Autores principales: | , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1994
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC205082/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8288551/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.176.2.535-539.1994 |
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