In Vivo Hexamerization and Characterization of the Arabidopsis AAA ATPase CDC48A Complex Using Förster Resonance Energy Transfer-Fluorescence Lifetime Imaging Microscopy and Fluorescence Correlation Spectroscopy

The Arabidopsis (Arabidopsis thaliana) AAA ATPase CDC48A was fused to cerulean fluorescent protein and yellow fluorescent protein. AAA ATPases like CDC48 are only active in hexameric form. Förster resonance energy transfer-based fluorescence lifetime imaging microscopy using CDC48A-cerulean fluoresc...

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Detalhes bibliográficos
Main Authors: Aker, José, Hesselink, Renske, Engel, Ruchira, Karlova, Rumyana, Borst, Jan Willem, Visser, Antonie J.W.G., de Vries, Sacco C.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society of Plant Biologists 2007
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2048723/
https://ncbi.nlm.nih.gov/pubmed/17693538
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.107.103986
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