Rapid Assembly of Multiple-Exon cDNA Directly from Genomic DNA

BACKGROUND: Polymerase chain reaction (PCR) is extensively applied in gene cloning. But due to the existence of introns, low copy number of particular genes and high complexity of the eukaryotic genome, it is usually impossible to amplify and clone a gene as a full-length sequence directly from the...

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Autores principales: An, Xiaoping, Lu, Jun, Huang, Jian-dong, Zhang, Baozhong, Liu, Dabin, Zhang, Xin, Chen, Jinhui, Zhou, Yusen, Tong, Yigang
Formato: Artigo
Lenguaje:Inglês
Publicado: Public Library of Science 2007
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Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2048664/
https://ncbi.nlm.nih.gov/pubmed/18000550
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0001179
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