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Rapid Assembly of Multiple-Exon cDNA Directly from Genomic DNA
BACKGROUND: Polymerase chain reaction (PCR) is extensively applied in gene cloning. But due to the existence of introns, low copy number of particular genes and high complexity of the eukaryotic genome, it is usually impossible to amplify and clone a gene as a full-length sequence directly from the...
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| 主要な著者: | , , , , , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Public Library of Science
2007
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2048664/ https://ncbi.nlm.nih.gov/pubmed/18000550 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0001179 |
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