Rapid Assembly of Multiple-Exon cDNA Directly from Genomic DNA

BACKGROUND: Polymerase chain reaction (PCR) is extensively applied in gene cloning. But due to the existence of introns, low copy number of particular genes and high complexity of the eukaryotic genome, it is usually impossible to amplify and clone a gene as a full-length sequence directly from the...

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Detalhes bibliográficos
Main Authors: An, Xiaoping, Lu, Jun, Huang, Jian-dong, Zhang, Baozhong, Liu, Dabin, Zhang, Xin, Chen, Jinhui, Zhou, Yusen, Tong, Yigang
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2007
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2048664/
https://ncbi.nlm.nih.gov/pubmed/18000550
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0001179
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