Rapid Assembly of Multiple-Exon cDNA Directly from Genomic DNA

BACKGROUND: Polymerase chain reaction (PCR) is extensively applied in gene cloning. But due to the existence of introns, low copy number of particular genes and high complexity of the eukaryotic genome, it is usually impossible to amplify and clone a gene as a full-length sequence directly from the...

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Bibliografiske detaljer
Main Authors: An, Xiaoping, Lu, Jun, Huang, Jian-dong, Zhang, Baozhong, Liu, Dabin, Zhang, Xin, Chen, Jinhui, Zhou, Yusen, Tong, Yigang
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2007
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2048664/
https://ncbi.nlm.nih.gov/pubmed/18000550
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0001179
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