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Screening for overlapping bacterial artificial chromosome clones by PCR analysis with an arbitrary primer

In this article, we used PCR analysis with arbitrary primers (AP-PCR) to screen for overlapping bacterial artificial chromosome (BAC) clones and assembly of contigs. A rice BAC library with three genome equivalents was used to prepare pooled BAC DNA. Twenty-two arbitrary primers were used to survey...

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Bibliographic Details
Main Authors: Xu, Jichen, Yang, Daichang, Domingo, Jessica, Ni, Junjian, Huang, Ning
Format: Artigo
Language:Inglês
Published: The National Academy of Sciences 1998
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC20435/
https://ncbi.nlm.nih.gov/pubmed/9576940
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