Screening for overlapping bacterial artificial chromosome clones by PCR analysis with an arbitrary primer

In this article, we used PCR analysis with arbitrary primers (AP-PCR) to screen for overlapping bacterial artificial chromosome (BAC) clones and assembly of contigs. A rice BAC library with three genome equivalents was used to prepare pooled BAC DNA. Twenty-two arbitrary primers were used to survey...

Full description

Saved in:
Bibliographic Details
Main Authors: Xu, Jichen, Yang, Daichang, Domingo, Jessica, Ni, Junjian, Huang, Ning
Format: Artigo
Language:Inglês
Published: The National Academy of Sciences 1998
Subjects:
Biological Sciences
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC20435/
https://ncbi.nlm.nih.gov/pubmed/9576940
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items