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Ex Vivo Monitoring of Antigen-Specific CD4(+) T Cells after Recall Immunization with Tetanus Toxoid

To monitor antigen-specific CD4(+) T cells during a recall immune response to tetanus toxoid (TT), a sequential analysis including ex vivo phenotyping and cytokine flow cytometry, followed by cloning and T-cell-receptor (TCR) spectratyping of cytokine-positive CD4(+) T cells, was performed. Grossly,...

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Detalles Bibliográficos
Main Authors: Barbey, Catherine, Pradervand, Estelle, Barbier, Nathalie, Spertini, François
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 2007
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2043311/
https://ncbi.nlm.nih.gov/pubmed/17634505
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CVI.00004-07
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