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Ex Vivo Monitoring of Antigen-Specific CD4(+) T Cells after Recall Immunization with Tetanus Toxoid
To monitor antigen-specific CD4(+) T cells during a recall immune response to tetanus toxoid (TT), a sequential analysis including ex vivo phenotyping and cytokine flow cytometry, followed by cloning and T-cell-receptor (TCR) spectratyping of cytokine-positive CD4(+) T cells, was performed. Grossly,...
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Main Authors: | , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
American Society for Microbiology (ASM)
2007
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2043311/ https://ncbi.nlm.nih.gov/pubmed/17634505 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CVI.00004-07 |
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