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Insights into the Mechanism of Flavoprotein-Catalyzed Amine Oxidation from Nitrogen Isotope Effects on the Reaction of N-Methyltryptophan Oxidase

The mechanism of N-methyltryptophan oxidase, a flavin-dependent amine oxidase from Escherichia coli, was studied using a combination of kinetic isotope effects and theoretical calculations. The (15)(k(cat)/K(m)) kinetic isotope effect for sarcosine oxidation is pH-dependent with a limiting value of...

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Detalhes bibliográficos
Main Authors: Ralph, Erik C., Hirschi, Jennifer S., Anderson, Mark A., Cleland, W. Wallace, Singleton, Daniel A., Fitzpatrick, Paul F.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2041825/
https://ncbi.nlm.nih.gov/pubmed/17542620
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi700482h
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