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Two-color selection for amplified co-production of proteins in mammalian cells

Structural and functional analyses for many mammalian systems depend on having abundant supplies of recombinant multi-protein complexes that can be produced best, or only, in mammalian cells. We present an efficient fluorescence marking procedure for establishing stable cell lines that overexpress t...

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Main Authors: Assur, Zahra, Schieren, Ira, Hendrickson, Wayne A., Mancia, Filippo
格式: Artigo
語言:Inglês
出版: 2007
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2041794/
https://ncbi.nlm.nih.gov/pubmed/17574435
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2007.04.025
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