Determining [(3)H]Thymidine Incorporation into Bacterioplankton DNA: Improvement of the Method by DNase Treatment

Determination of [(3)H] thymidine incorporation into bacterial DNA versus other macromolecules is usually achieved by NaOH and hot trichloroacetic acid hydrolysis. This procedure was found not to be specific enough. An alternative method founded on DNase treatment is proposed. Under the new method,...

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Detalles Bibliográficos

Publicado en:	Appl Environ Microbiol
Principais autores:	Servais, P., Martinez, J., Billen, G., Vives-Rego, J.
Formato:	Artigo
Idioma:	Inglês
Publicado:	American Society for Microbiology (ASM) 1987
Assuntos:	General Microbial Ecology
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC204039/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16347424/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.53.8.1977-1979.1987
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