Determining [(3)H]Thymidine Incorporation into Bacterioplankton DNA: Improvement of the Method by DNase Treatment

Determination of [(3)H] thymidine incorporation into bacterial DNA versus other macromolecules is usually achieved by NaOH and hot trichloroacetic acid hydrolysis. This procedure was found not to be specific enough. An alternative method founded on DNase treatment is proposed. Under the new method,...

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Publié dans:	Appl Environ Microbiol
Auteurs principaux:	Servais, P., Martinez, J., Billen, G., Vives-Rego, J.
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1987
Sujets:	General Microbial Ecology
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC204039/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16347424/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.53.8.1977-1979.1987
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