Determining [(3)H]Thymidine Incorporation into Bacterioplankton DNA: Improvement of the Method by DNase Treatment

Determination of [(3)H] thymidine incorporation into bacterial DNA versus other macromolecules is usually achieved by NaOH and hot trichloroacetic acid hydrolysis. This procedure was found not to be specific enough. An alternative method founded on DNase treatment is proposed. Under the new method,...

ver descrição completa

Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Servais, P., Martinez, J., Billen, G., Vives-Rego, J.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1987
Assuntos:	General Microbial Ecology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC204039/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16347424/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.53.8.1977-1979.1987
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!