Cloning, Expression, and Purification of Glutamine Synthetase from Clostridium acetobutylicum

A glutamine synthetase (GS) gene, glnA, from the gram-positive obligate anaerobe Clostridium acetobutylicum was cloned on recombinant plasmid pHZ200 and enabled Escherichia coli glnA deletion mutants to utilize (NH(4))(2)SO(4) as a sole source of nitrogen. The cloned C. acetobutylicum gene was expre...

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Bibliografiske detaljer

Udgivet i:	Appl Environ Microbiol
Principais autores:	Usdin, Karen P., Zappe, Harold, Jones, David T., Woods, David R.
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1986
Fag:	Genetics and Molecular Biology
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203548/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16347143/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.52.3.413-419.1986
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