Cloning, Expression, and Purification of Glutamine Synthetase from Clostridium acetobutylicum
A glutamine synthetase (GS) gene, glnA, from the gram-positive obligate anaerobe Clostridium acetobutylicum was cloned on recombinant plasmid pHZ200 and enabled Escherichia coli glnA deletion mutants to utilize (NH(4))(2)SO(4) as a sole source of nitrogen. The cloned C. acetobutylicum gene was expre...
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| Publicado no: | Appl Environ Microbiol |
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| Principais autores: | , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1986
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203548/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16347143/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.52.3.413-419.1986 |
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