Modified plasmid isolation method for Clostridium perfringens and Clostridium absonum.

A rapid plasmid isolation procedure for Clostridium perfringens and C. absonum is described. The ratio of culture volume to lysis buffer volume was found to be crucial for efficient plasmid isolation. The method can be scaled up, without difficulty, for large-scale plasmid preparation. IMAGES:

Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Roberts, I, Holmes, W M, Hylemon, P B
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1986
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203446/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2873789/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.52.1.197-199.1986
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