Modified plasmid isolation method for Clostridium perfringens and Clostridium absonum.

A rapid plasmid isolation procedure for Clostridium perfringens and C. absonum is described. The ratio of culture volume to lysis buffer volume was found to be crucial for efficient plasmid isolation. The method can be scaled up, without difficulty, for large-scale plasmid preparation. IMAGES:

Xehetasun bibliografikoak

Argitaratua izan da:	Appl Environ Microbiol
Egile Nagusiak:	Roberts, I, Holmes, W M, Hylemon, P B
Formatua:	Artigo
Hizkuntza:	Inglês
Argitaratua:	American Society for Microbiology (ASM) 1986
Gaiak:	Research Article
Sarrera elektronikoa:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203446/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2873789/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.52.1.197-199.1986
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