Modified plasmid isolation method for Clostridium perfringens and Clostridium absonum.

A rapid plasmid isolation procedure for Clostridium perfringens and C. absonum is described. The ratio of culture volume to lysis buffer volume was found to be crucial for efficient plasmid isolation. The method can be scaled up, without difficulty, for large-scale plasmid preparation. IMAGES:

Podrobná bibliografie

Vydáno v:	Appl Environ Microbiol
Hlavní autoři:	Roberts, I, Holmes, W M, Hylemon, P B
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	American Society for Microbiology (ASM) 1986
Témata:	Research Article
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203446/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2873789/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.52.1.197-199.1986
Tagy:	Přidat tag Žádné tagy, Buďte první, kdo vytvoří štítek k tomuto záznamu!