Processing of long-stored archival cervical smears for human papillomavirus detection by the polymerase chain reaction.

The efficiency of a freeze-thaw method, a proteinase K/Tween 20 lysis method and a guanidinium isothiocyanate/silica beads method for DNA extraction from fixed and Papanicolaou-stained cells from the cervical cancer cell line Siha was measured by beta-globin polymerase chain reaction (PCR). The GTC/...

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Autors principals: de Roda Husman, A. M., Snijders, P. J., Stel, H. V., van den Brule, A. J., Meijer, C. J., Walboomers, J. M.
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group 1995
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2033993/
https://ncbi.nlm.nih.gov/pubmed/7543772
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