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A quantitative polymerase chain reaction-enzyme immunoassay for accurate measurements of human papillomavirus type 16 DNA levels in cervical scrapings

A quantitative polymerase chain reaction-enzyme immunoassay (Q-PCR-EIA) was developed to measure the amount of human papillomavirus (HPV) 16 DNA per genome equivalent in cervical scrapings. The quantitative approach was based on a combined competitive PCR for both HPV 16, using the general primer GP...

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Detalhes bibliográficos
Main Authors: Jacobs, M V, Walboomers, J M M, Beek, J van, Voorhorst, F J, Verheijen, R H M, Meijer, C J L M, Brule, A J C van den, Helmerhorst, ThJ M, Snijders, P J F
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 1999
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2374354/
https://ncbi.nlm.nih.gov/pubmed/10487621
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/sj.bjc.6690659
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