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Rapid detection of human papillomavirus in cervical scrapes by combined general primer-mediated and type-specific polymerase chain reaction.
A two-step polymerase chain reaction (PCR) procedure was used as a new screening strategy for the detection of human papillomavirus (HPV) genotypes in cervical scrapes omitting prior DNA extraction. Sample preparation consisted of a freeze-thaw step followed by boiling the cells before the PCR mixtu...
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| Published in: | J Clin Microbiol |
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| Main Authors: | , , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
American Society for Microbiology (ASM)
1990
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC268265/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2177751/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.28.12.2739-2743.1990 |
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