Rapid detection of human papillomavirus in cervical scrapes by combined general primer-mediated and type-specific polymerase chain reaction.

A two-step polymerase chain reaction (PCR) procedure was used as a new screening strategy for the detection of human papillomavirus (HPV) genotypes in cervical scrapes omitting prior DNA extraction. Sample preparation consisted of a freeze-thaw step followed by boiling the cells before the PCR mixtu...

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Bibliographic Details
Published in:J Clin Microbiol
Main Authors: van den Brule, A J, Meijer, C J, Bakels, V, Kenemans, P, Walboomers, J M
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1990
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC268265/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2177751/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jcm.28.12.2739-2743.1990
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