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Rapid detection of human papillomavirus in cervical scrapes by combined general primer-mediated and type-specific polymerase chain reaction.

A two-step polymerase chain reaction (PCR) procedure was used as a new screening strategy for the detection of human papillomavirus (HPV) genotypes in cervical scrapes omitting prior DNA extraction. Sample preparation consisted of a freeze-thaw step followed by boiling the cells before the PCR mixtu...

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Autors principals: van den Brule, A J, Meijer, C J, Bakels, V, Kenemans, P, Walboomers, J M
Format: Artigo
Idioma:Inglês
Publicat: 1990
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC268265/
https://ncbi.nlm.nih.gov/pubmed/2177751
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