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Molecular Cloning and Expression of a Xylanase Gene from Bacillus polymyxa in Escherichia coli

Genomic fragments of Bacillus polymyxa derived from separate and complete digestion by EcoRI, HindIII, and BamHI were ligated into the corresponding sites of pBR322, and the resulting chimeric plasmids were transformed into Escherichia coli. Of 6,000 transformants screened, 1 (pBPX-277) produced a c...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Principais autores: Yang, Robert C. A., MacKenzie, C. Roger, Bilous, Doris, Seligy, Verner L., Narang, Saran A.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1988
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC202590/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16347597/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.54.4.1023-1029.1988
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