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Molecular Cloning and Expression of a Xylanase Gene from Bacillus polymyxa in Escherichia coli

Genomic fragments of Bacillus polymyxa derived from separate and complete digestion by EcoRI, HindIII, and BamHI were ligated into the corresponding sites of pBR322, and the resulting chimeric plasmids were transformed into Escherichia coli. Of 6,000 transformants screened, 1 (pBPX-277) produced a c...

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Publicat a:Appl Environ Microbiol
Autors principals: Yang, Robert C. A., MacKenzie, C. Roger, Bilous, Doris, Seligy, Verner L., Narang, Saran A.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 1988
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC202590/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16347597/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.54.4.1023-1029.1988
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