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Hyperexpression of a Bacillus circulans xylanase gene in Escherichia coli and characterization of the gene product.

A 4.0-kilobase (kb) fragment of Bacillus circulans genomic DNA inserted into pUC19 and encoding endoxylanase activity was subjected to a series of subclonings. A 1.0-kb HindIII-HincII subfragment was found to code for xylanase activity. Maximum expression levels were observed with a subclone that co...

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Bibliografiske detaljer
Udgivet i:Appl Environ Microbiol
Principais autores: Yang, R C, MacKenzie, C R, Bilous, D, Narang, S A
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1989
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC184275/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2667461/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.55.5.1192-1195.1989
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