Hyperexpression of a Bacillus circulans xylanase gene in Escherichia coli and characterization of the gene product.
A 4.0-kilobase (kb) fragment of Bacillus circulans genomic DNA inserted into pUC19 and encoding endoxylanase activity was subjected to a series of subclonings. A 1.0-kb HindIII-HincII subfragment was found to code for xylanase activity. Maximum expression levels were observed with a subclone that co...
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| Publicat a: | Appl Environ Microbiol |
|---|---|
| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1989
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC184275/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2667461/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.55.5.1192-1195.1989 |
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