Some characteristics of a proteinase from a thermophilic Bacillus sp. expressed in Escherichia coli: comparison with the native enzyme and its processing in E. coli and in vitro.
Proteinase Ak.1 was produced during the stationary phase of Bacillus sp. Ak.1 cultures. It is a serine proteinase with a pI of 4.0, and the molecular mass was estimated to be 36.9 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme was stable at 60 and 70 degrees...
Na minha lista:
| Udgivet i: | Appl Environ Microbiol |
|---|---|
| Principais autores: | , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1993
|
| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC202256/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8476291/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.59.4.1168-1175.1993 |
| Tags: |
Ingen Tags, Vær først til at tagge denne postø!
|