Rapid isolation and sequencing of purified plasmid DNA from Bacillus subtilis.

We report two methods for isolation of plasmid DNA from the gram-positive bacterium Bacillus subtilis. The protoplast alkaline lysis procedure was developed for general use, and the protoplast alkaline lysis magic procedure was developed for isolation of DNA for sequencing. Both procedures yielded l...

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Detalles Bibliográficos

Publicado en:	Appl Environ Microbiol
Autores principales:	Voskuil, M I, Chambliss, G H
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	American Society for Microbiology (ASM) 1993
Materias:	Research Article
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC202251/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8476287/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.59.4.1138-1142.1993
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