Rapid isolation and sequencing of purified plasmid DNA from Bacillus subtilis.

We report two methods for isolation of plasmid DNA from the gram-positive bacterium Bacillus subtilis. The protoplast alkaline lysis procedure was developed for general use, and the protoplast alkaline lysis magic procedure was developed for isolation of DNA for sequencing. Both procedures yielded l...

Ausführliche Beschreibung

Bibliografische Detailangaben

Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Voskuil, M I, Chambliss, G H
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1993
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC202251/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8476287/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.59.4.1138-1142.1993
Tags:	Tag hinzufügen Keine Tags, Fügen Sie das erste Tag hinzu!