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Rapid isolation and sequencing of purified plasmid DNA from Bacillus subtilis.

We report two methods for isolation of plasmid DNA from the gram-positive bacterium Bacillus subtilis. The protoplast alkaline lysis procedure was developed for general use, and the protoplast alkaline lysis magic procedure was developed for isolation of DNA for sequencing. Both procedures yielded l...

詳細記述

保存先:
書誌詳細
主要な著者: Voskuil, M I, Chambliss, G H
フォーマット: Artigo
言語:Inglês
出版事項: 1993
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC202251/
https://ncbi.nlm.nih.gov/pubmed/8476287
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