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Rapid polymerase chain reaction method for detection of Vibrio cholerae in foods.

The polymerase chain reaction was used to selectively amplify sequences within the cholera toxin operon from Vibrio cholerae O1. Oysters, crabmeat, shrimp, and lettuce were seeded with V. cholerae and then homogenized or washed with alkaline peptone water, followed by short-term (6- to 8-h) enrichme...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Principais autores: Koch, W H, Payne, W L, Wentz, B A, Cebula, T A
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1993
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC202143/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8434922/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.59.2.556-560.1993
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