Efficient Transformation of the Cephamycin C Producer Nocardia lactamdurans and Development of Shuttle and Promoter-Probe Cloning Vectors

A high transformation efficiency (1 × 10(5) to 7 × 10(5) transformants per μg of DNA) of Nocardia lactamdurans LC411 was obtained by direct treatment of mycelium with polyethylene glycol 1000 and cesium chloride. A variety of vectors from Streptomyces lividans, Brevibacterium lactofermentum, Rhodoco...

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Publié dans:	Appl Environ Microbiol
Auteurs principaux:	Kumar, C. Vasant, Coque, Juan-Jose R., Martín, Juan F.
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1994
Sujets:	Genetics and Molecular Biology
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC201940/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16349436/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.60.11.4086-4093.1994
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