Efficient Transformation of the Cephamycin C Producer Nocardia lactamdurans and Development of Shuttle and Promoter-Probe Cloning Vectors
A high transformation efficiency (1 × 10(5) to 7 × 10(5) transformants per μg of DNA) of Nocardia lactamdurans LC411 was obtained by direct treatment of mycelium with polyethylene glycol 1000 and cesium chloride. A variety of vectors from Streptomyces lividans, Brevibacterium lactofermentum, Rhodoco...
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| Wydane w: | Appl Environ Microbiol |
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| Główni autorzy: | , , |
| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
American Society for Microbiology (ASM)
1994
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC201940/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16349436/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.60.11.4086-4093.1994 |
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