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One step construction of PCR mutagenized libraries for genetic analysis by recombination cloning

Recombination cloning encompasses a set of technologies that transfer gene sequences between vectors through site-specific recombination. Due in part to the instability of linear DNA in bacteria, both the initial capture and subsequent transfer of gene sequences is often performed using purified rec...

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Hlavní autoři: Khalil, Al-Muataz, Julius, Jeffrey A., Bachant, Jeff
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2007
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2018627/
https://ncbi.nlm.nih.gov/pubmed/17702758
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm583
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