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Development of a method for detection of enteroviruses in shellfish by PCR with poliovirus as a model.

The application of the PCR to complex samples is hindered by amplification inhibitors. We describe a reverse transcription-PCR-based method capable of inhibitor removal for the detection of enteroviruses in shellfish. Initial virus extraction stages based on a modified polyethylene glycol precipitat...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Principais autores: Lees, D N, Henshilwood, K, Doré, W J
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1994
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC201755/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7521997/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.60.8.2999-3005.1994
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