Development of a method for detection of enteroviruses in shellfish by PCR with poliovirus as a model.
The application of the PCR to complex samples is hindered by amplification inhibitors. We describe a reverse transcription-PCR-based method capable of inhibitor removal for the detection of enteroviruses in shellfish. Initial virus extraction stages based on a modified polyethylene glycol precipitat...
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| Izdano u: | Appl Environ Microbiol |
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| Glavni autori: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
American Society for Microbiology (ASM)
1994
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC201755/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7521997/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.60.8.2999-3005.1994 |
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