Development of a method for detection of enteroviruses in shellfish by PCR with poliovirus as a model.

The application of the PCR to complex samples is hindered by amplification inhibitors. We describe a reverse transcription-PCR-based method capable of inhibitor removal for the detection of enteroviruses in shellfish. Initial virus extraction stages based on a modified polyethylene glycol precipitat...

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Pubblicato in:	Appl Environ Microbiol
Autori principali:	Lees, D N, Henshilwood, K, Doré, W J
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1994
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC201755/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7521997/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.60.8.2999-3005.1994
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