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Performance of plate-based cytokine flow cytometry with automated data analysis
BACKGROUND: Cytokine flow cytometry (CFC) provides a multiparameter alternative to ELISPOT assays for rapid quantitation of antigen-specific T cells. To increase the throughput of CFC assays, we have optimized methods for stimulating, staining, and acquiring whole blood or PBMC samples in 96-well or...
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Hoofdauteurs: | , , , , , , , , , |
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Formaat: | Artigo |
Taal: | Inglês |
Gepubliceerd in: |
BioMed Central
2003
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Onderwerpen: | |
Online toegang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC200973/ https://ncbi.nlm.nih.gov/pubmed/12952557 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2172-4-9 |
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